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Vol 58(2024) N 6 p. 1063-1073; DOI 10.1134/S0026893324700560  S.V. Revtovich1*, V.V. Kulikova1, V.S. Koval1, A.D. Lyfenko1, V.A. Kazakov2, A.S. Chernov2, G.B. Telegin2, A.S. Zemskaya1, N.V. Anufrieva1, E.A. Morozova1, P.N. Solyev1 Toxicity Study of the Pharmacological Pair of Encapsulated Citrobacter freundii C115H Methionine γ-Lyase/Methiin 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia *svetla21@mail.ru Received - 2024-04-12; Revised - 2024-05-29; Accepted - 2024-05-31 Acute and subchronic toxicity of the pharmacological pair based on encapsulated Citrobacter freundii C115H methionine γ-lyase enzyme/prodrug (methiin) was studied in female ICR mice. The drug showed a weak/moderate dose-dependent hepatotoxic effect. Most of the changes in liver morphology identified were insignificant or mild deviations from the norm. Long-term use of a single therapeutic dose per mouse of 1.5 U C. freundii C115H methionine γ-lyase @ (PEG-P(Asp) 70/PLL70)-PIC-some/2 mg methiin led to a slight decrease in the weight of animals without obvious signs of intoxication. A quarter of the animals in this group had no deviations from the norm in liver morphology. No nephrotoxic effect was found in any study group. methionine γ-lyase, methiin, pharmacological pair, prodrug, acute toxicity, subchronic toxicity |
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