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Vol 58(2024) N 4 p. 700-707; DOI 10.1134/S0026893324700286  J.V. Nikolenko1.2, M.M. Kurshakova2, D.V. Kopytova2, Y.A. Vdovina2, N.E. Vorobyova1, A.N. Krasnov1* The Drosophila Zinc Finger Proteins Aefl and CG10543 Are Co-Localized with SAGA, SWI/SNF, and ORC Complexes on Gene Promoters and Involved in Transcription Regulation 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia *krasnov@genebiology.ru Received - 2023-12-03; Revised - 2023-12-11; Accepted - 2023-12-12 In previous studies, we purified the DUB-module of the Drosophila SAGA complex and showed that a number of zinc proteins interact with it, including Aef1 and CG10543. In this work, we conducted a genome-wide study of the Aef1 and CG10543 proteins and showed that they are localized predominantly on the promoters of active genes. The binding sites of these proteins co-localize with the SAGA and dSWI/SNF chromatin modification and remodeling complexes, as well as with the ORC replication complex. It has been shown that the Aef1 and CG10543 proteins are involved in the regulation of the expression of some genes on the promoters of which they are located. Thus, the Aef1 and CG10543 proteins are new participants in the cell transcriptional network and co-localize with the main transcription and replication complexes of Drosophila. Aef1, CG10543, zinc fingers, transcription regulation, DUB-module, complex SAGA, SWI/SNF, ORC |
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