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Vol 43(2009) N 6 p. 1004-1007; I.Z. Shukshina1, E.E. Minyat2 Effect of aminoglycoside antibiotics on the conformation of the unpaired loop adenine of avian leucosis virus RNA as revealed using 2-aminopurine fluorescence 1Moscow Institute of Physics and Technology, Dolgoprudny, Moscow oblast, 141700, Russia2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia Received - 2009-02-20; Accepted - 2009-04-10 The fluorescent properties of 2-aminopurine (2-AP) incorporated in an RNA sequence are used to study the structural dynamics and local changes of the retroviral RNA structure. Using 2-AP, the conformational states of the unpaired loop adenine in avian leucosis virus RNA were studied upon its interaction with aminoglycoside antibiotics. The intensity of 2-AP fluorescence in the monomeric RNA hairpin was higher than in both RNA dimers. The intensity of fluorescence in the extended dimer was significantly lower than in the kissing loop dimer. The finding was be explained by the fact that stacking contacts in the extended dimer produce a more compact loop structure than in the kissing loop dimer. When the binding of aminogycoside antibiotics with the kissing loop dimer RNA was analyzed, only tobramycin increased the intensity of 2-AP fluorescence almost threefold. The results showed that 2-AP fluorescence is suitable for detecting local changes in complexes of retroviral RNA with ligands. RNA, retroviruses, structure, dimerization, fluorescence, 2-aminopurine, aminoglycoside antibiotics |
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