2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 48(2014) N 3 p. 424-431; DOI 10.1134/S002689331403008X Full Text

A.V. Ivanov, A.A. Malygin, G.G. Karpova*

Common features in arrangements of ribosomal protein S26e binding sites on its own pre-mRNA and the 18S rRNA

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090, Russia

Received - 2013-11-19; Accepted - 2013-12-19

Human ribosomal protein (rp) S26e is known to bind to the first intron of its pre-mRNA and, thus, inhibit mRNA splicing. In this work, the hydroxyl radical footprinting was applied for the detailed mapping of the rpS26e binding site on the RNA transcript that corresponds to the rpS26e pre-mRNA fragment containing the first intron flanked by the sequences of the first exon and a part of the second exon. Nucleotides protected from the hydroxyl radical attack in the presence of rpS26e were identified. Most of them were found in the region of the 3' splice site of the first intron within the purine-rich sequence, which forms a loop between two helices in the predicted secondary structure of the rpS26e pre-mRNA fragment, and the remaining nucleotides are located near the 5' splice site. A comparison of the arrangements of the rpS26e binding sites on the pre-mRNA and 18S rRNA secondary structures revealed similar elements in the organization of these sites. It was found that both sites contained a uniform structural motif, namely an extended purine-rich loop between two helices, which could be recognized by rpS26e upon binding to these RNAs. The results shed light on the structural aspects of the RNA-protein interactions, which underlie the autoregulation of the human RPS26e gene expression at the splicing step.

human ribosomal protein S26e, pre-mRNA of ribosomal protein S26e, hydroxyl radical footprinting, RNA-protein interaction, 5'- and 3'-sites of splicing