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Vol 58(2024) N 3 p. 527-533; DOI 10.1134/S0026893324700158  A.A. Kudryavtseva1, A.V. Vlasov1,2,3, E.V. Zinovev1, D.D. Yanovskaya4, A.A. Utkina1, S.M. Rastorguev5, I.V. Manukhov1,2* ArdA Protein Specificity against Type I Restriction-Modification Systems 1Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow oblast, 141707 Russia2Russian Biotechnological University, Moscow, 125080 Russia 3Joint Institute for Nuclear Research, Dubna, Moscow oblast, 141980 Russia 4Skolkovo Institute of Science and Technology, Moscow, 143028 Russia 5Pirogov Russian National Research Medical University, Moscow, 117997Russia *manukhovi@mail.ru Received - 2023-11-11; Revised - 2023-12-06; Accepted - 2023-12-06 The ArdA DNA-mimic antirestriction proteins inhibit type I restriction-modification (RMI) systems by binding instead of DNA to RMI. The ArdA specificity to DNA methylation sites recognized by RMI complexes remains poorly understood; i.e., it is unclear whether a particular DNA site is mimicked by ArdA. The ardA genes were cloned from three Gram-positive bacteria: Agrobacterium tumefaciens, Pseudomonas monteilii, and Xanthomonas sp. Antirestriction activities of their products were tested against three Escherichia coli RMI systems differing in DNA recognition/methylation sites. Although similar structures were predicted for the ArdA proteins, the strong specificity to three RMI systems was observed. The results indicate that specific DNA sites may be imitated by DNA mimic ArdA proteins. antirestriction, RMI, ArdA, DNA mimicry |
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