2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 57(2023) N 5 p. 848-866; DOI 10.1134/S0026893323050060 Full Text

L.A. Koval1, E.N. Proshkina1, N.V. Zemskaya1, I.A. Solovev1,2, E.V. Schegoleva1, M.V. Shaposhnikov1, A.A. Moskalev1,3,4*

Drosophila melanogaster Lifespan Is Regulated by nejire Gene Expression in Peripheral Tissues and Nervous System

1Institute of Biology, Komi Scientific Center, Ural Branch, Russian Academy of Sciences, Syktyvkar, 167982 Russia
2Pitirim Sorokin Syktyvkar State University, Syktyvkar, 167001 Russia
3Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
4Russian Clinical Research Center for Gerontology, Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, 129226 Russia

Received - 2022-12-25; Revised - 2023-03-27; Accepted - 2023-03-28

Histone acetyltransferases of the CBP/p300 family are involved in transcriptional regulation and many biological processes (cell proliferation and differentiation, development, and regulation of the stress response and metabolism). Overexpression and knockdown of the nejire (nej) gene (codes for an ortholog of human CBP/p300 proteins) in various tissues (the fat body, intestine, and nervous system) and at various stages of the life cycle (throughout all developmental stages or in adulthood only) were tested for effect on lifespan in the fruit fly Drosophila melanogaster. The activation of nej exerted a positive or a negative effect on the lifespan, depending on the induction mode and the sex. A 6-15% greater lifespan was observed in females with conditional overexpression of nej in the intestine and constitutive overexpression of nej in the nervous system. A decrease (to 44%) or lack of significant changes in lifespan was detected in all other cases observed. In addition, stress response genes (Sod1, Gadd45, Hsp27, Hsp68, and Hif1) were regulated by nej activation. nej knockdown caused a pronounced negative effect on the D. melanogaster lifespan in most variants of the experiment.

histone acetyltransferase, CBP/p300, nejire, lifespan, stress resistance, Drosophila melanogaster