2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 48(2014) N 3 p. 384-389; DOI 10.1134/S002689331403011X Full Text

I.V. Kondakova*, L.V. Spirina, V.D. Koval, E.E. Shashova, E.L. Choinzonov, E.V. Ivanova, L.A. Kolomiets, A.L. Chernyshova, E.M. Slonimskaya, E.A. Usynin, S.G. Afanas'ev

Chymotrypsin-Like Activity and Subunit Composition of Proteasomes in Human Cancers

Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, 634009, Russia

Received - 2013-11-19; Accepted - 2013-12-23

The activity of the proteasome, a polyfunctional enzymatic complex, is known to undergo changes during cancer development. This phenomenon is probably caused by the changes in subunit composition of proteasomes. In this work, we studied the chymotrypsin-like activity of proteasomes; their subunit composition; and their association in breast cancer, head and neck squamous cell carcinoma, endometrial cancer, renal cancer, bladder cancer, stomach cancer, and colorectal cancer. The increase in proteasome activity was revealed in most cancer tissues compared with adjacent tissues, except for the renal cell carcinoma. Changes in proteasome activity in cancer tissues compared with correspondent normal tissues observed in combination with an increased expression of immune subunits and/or proteasome activator PA28 associated with activity of 20S proteasome. In breast cancer, head and neck squamous cell carcinoma, bladder cancer, stomach cancer, and colorectal cancer, we additionally found the higher expression of Rpt6 subunit of the 19S-subunit in 26S proteasome. Correlations between chymotrypsin-like proteasome activity and subunit expressions were found in human cancer tissues. Thus, we suggest that proteasome activation and changes in its subunit composition play an important role in cancer pathogenesis.

proteasomes, chymotrypsin-like activity, subunit composition of proteasomes, human cancer