Vol 56(2022) N 4 p. 533-542; DOI 10.1134/S0026893322040070
L. Ma1, D.H. Li1, Z. Xu1*
HECTD2 Represses Cell Proliferation in Colorectal Cancer through Driving Ubiquitination and Degradation of LPCAT11Department of General Surgery, Qingdao Municipal Hospital, Qingdao University, 266000 China
Received - 2021-11-09; Revised - 2021-12-29; Accepted - 2021-12-29
Colorectal cancer (CRC) is a malignancy featured by a poor overall survival and a high recurrence rate, whereas the biomarkers for CRC remain to be investigated. Herein, it was found that lysophosphatidylcholine acyltransferase 1 (LPCAT1) was highly expressed in CRC, and LPCAT1 overexpression significantly promoted CRC cell proliferation, while it was reversed by LPCAT1 depletion. In addition, HECT domain-containing 2 (HECTD2) protein was determined as a post-translational mediator of LPCAT1 because HECTD2 co-immunoprecipitated with high ubiquitinated LPCAT1. Furthermore, upregulated LPCAT1 rescued the impairment of CRC cell proliferation caused by HECTD2 overexpression. In conclusion, our findings supported HECTD2/LPCAT1 axis as a potential prognostic biomarker in CRC.
colorectal cancer, HECTD2, LPCAT1, ubiquitination, cell proliferation