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Vol 56(2022) N 3 p. 463-468; DOI 10.1134/S0026893322030050 Full Text

A.A. Dolskiy1, I.V. Grishchenko1, S.A. Bodnev1, A.A. Nazarenko1, A.M. Smirnova1, A.K. Matveeva1, L.E. Bulychev1, A.S. Ovchinnikova1, T.V. Tregubchak1, A.V. Zaykovskaya1, I.R. Imatdinov1, O.V. Pyankov1, E.V. Gavrilova1, R.A. Maksyutov1, D.V. Yudkin1*

Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines

1State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, Koltsovo, Novosibirsk Region, 630559 Russia

*yudkin_dv@vector.nsc.ru
Received - 2021-11-22; Revised - 2021-12-13; Accepted - 2021-12-13

Coronaviridae is a family of single-stranded RNA (ssRNA) viruses that can cause diseases with high mortality rates. SARS-CoV-1 and MERS-CoV appeared in 2002-2003 and 2012, respectively. A novel coronavirus, SARS-CoV-2, emerged in 2019 in Wuhan (China) and has caused more than 5 million deaths in worldwide. The entry of SARS-CoV-1 into the cell is due to the interaction of the viral spike (S) protein and the cell protein, angiotensin-converting enzyme 2 (ACE2). After infection, virus assembly occurs in Golgi apparatus-derived vesicles during exocytosis. One of the possible participants in this process is LAMP1 protein. We established transgenic Vero cell lines with increased expression of human LAMP1 gene and evaluated SARS-CoV-1 and SARS-CoV-2 production. An increase in the production of both viruses in LAMP1-expressing cells when compared with Vero cells was observed, especially in the presence of trypsin during infection. From these results it can be assumed that LAMP1 promotes SARS-CoV-1 and SARS-CoV-2 production due to enhanced exocytosis.

SARS-CoV-1, SARS-CoV-2, LAMP1, COVID-19, Vero, transgenic cells



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