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Vol 56(2022) N 3 p. 453-462; DOI 10.1134/S0026893322030153 Full Text

Y.C. Zhang1,2, J.W. Wang2,3, Y. Wu2,3, Q. Tao4, F.F. Wang2,3, N. Wang1,2, X.R. Ji1,2, Y.G. Li4, S. Yu1,2*, J. Z. Zhang1,2,3,5**

Multimodal Magnetic Resonance and Fluorescence Imaging of the Induced Pluripotent Stem Cell Transplantation in the Brain

1University of Science and Technology of China, Hefei, 230026 P.R. China
2Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163 P.R. China
3Zhengzhou Institute of Engineering and Technology Affiliated with SIBET, Zhengzhou, 450001 P.R. China
4Department of Radiology, The First Affiliated Hospital of Soochow University, Suzhou, 215100 P.R. China
5Tianjin Guokeyigong Science and Technology Development Company Limited, Tianjin, 300399 P.R. China

*yush@sibet.ac.cn
**zhangjz@sibet.ac.cn
Received - 2021-09-27; Revised - 2021-11-11; Accepted - 2021-11-25

The understanding of the engrafted cell behaviors such as the survival, growth and distribution is the prerequisite to optimize cell therapy, and a multimodal imaging at both anatomical and molecular levels is designed to achieve this goal. We constructed a lentiviral vector carrying genes of ferritin heavy chain 1 (FTH1), near-infrared fluorescent protein (iRFP) and enhanced green fluorescent protein (egfp), and established the induced pluripotent stem cells (iPSCs) culture stably expressing these three reporter genes. These iPSCs showed green and near-infrared fluorescence as well as the iron uptake capacity in vitro. After transplanted the labeled iPSCs into the rat brain, the engrafted cells could be in vivo imaged using magnetic resonance imaging (MRI) and near-infrared fluorescent imaging (NIF) up to 60 days at the anatomical level. Moreover, these cells could be detected using EGFP immunostaining and Prussian blue stain at the cellular level. The developed approach provides a novel tool to study behaviors of the transplanted cells in a multi-modal way, which will be valuable for the effectiveness and safety evaluation of cell therapy.

multimodal imaging, magnetic resonance imaging, fluorescent imaging, cell therapy, brain



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