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Vol 56(2022) N 3 p. 443-452; DOI 10.1134/S0026893322030116 Full Text

Kum Chol Ri1,2*, Myong Ryong Ri1, Kwang Hun Kim3, Sun Il Choe4, Ju Hua Ri1, Ji Hyon Kim1, Jong Ho Ri1

KLF6 Super-enhancer Regulates Cell Proliferation by Recruiting GATA2 and SOX10 in Human Hepatoma Cells

1Life Science Department, University of Science, Pyongyang, 999095 Democratic People's Republic of Korea
2School of Management, Harbin Institute of Technology, Harbin, Heilongjiang, 150001 China
3Department of Engineering Machine, Pyongyang University of Mechanical Engineering, Pyongyang, 950003 Democratic People's Republic of Korea
4Life Science Department, Kim Il Sung University, Pyongyang, 999093 Democratic People's Republic of Korea

*RKCHIT@hit.edu.cn
Received - 2021-09-01; Revised - 2021-11-11; Accepted - 2021-11-16

Super-enhancer consists of a large cluster of transcription enhancers that regulates the expression of genes playing an important role in the growth and development of malignant tumors. Recently, several attempts for the identification of super-enhancers have been made, but their functional role in tumor cells remains unclear. This paper aims at elucidating the functional properties of KLF6 super-enhancer related to the growth regulation of HepG2 cells, in relation to transcription factors (TFs). First, some TFs specifying KLF6 super enhancer were identified using CRISPR/Cas9 system and siRNA. Then, their effects on the expression of the target gene KLF6 were assessed. Last, their influence on the proliferation of tumor cells was considered using the MTT method. The study shows that the active enhancers of KLF6 super-enhancer recruit GATA2 and SOX10 TFs to control the expression of the target gene, KLF6. Our findings suggest that the activity of KLF6 super-enhancer is regulated by two TFs (GATA2 and SOX10), and its targeting may be a potential therapeutic strategy for the liver cancer therapy.

super-enhancer, KLF6, CRISPR/Cas9, GATA2, SOX10, siRNA



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