2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 48(2014) N 3 p. 377-383; DOI 10.1134/S0026893314030029 Full Text

L.N. Astakhova*, O.G. Zatsepina, M.B. Evgen'ev, D.G. Garbuz

Comparative Analysis of Effectiveness of Heat-Shock Promoters in Two Diptera Species

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

Received - 2013-10-16; Accepted - 2013-12-21

Heat-shock proteins (Hsp) provide the cellular and full-body adaptation of animals to various adverse environmental conditions. The hsp70 is believed to play a major role in the biological adaptation of all organisms studied so far. In all animals the regulatory regions of the heat shock genes studied include several conservative promoter elements HSEs (heat shock elements) that are needed to bind heat-shock transcription factor (HSF). The promoter regions of the hsp70 genes are extremely conserved and, hence, it has been generally accepted that they are universal and can operate in species that belong to different phyla. In the present work, we performed a comparative analysis that revealed characteristic differences in the hsp70 promoters of two Diptera species, i.e., Drosophila melanogaster and highly thermotolerance soldier fly Stratiomys singularior. We measured promoter activity in D. melanogaster cell culture exploring in vitro luciferase reporter assay. The analysis demonstrated significantly higher strength of D. melanogaster promoters despite that comparable numbers of HSEs were present in both species. These drastic differences in the promoter strength are probably due to the absence of GAF-binding sites, which are necessary for the efficient functioning of D. melanogaster hsp70 promoters. In contrast, the comparison of hsp83 promoters isolated from these two species has not shown significant differences. Our results demonstrate existence of different evolutionary trends in the regulation of the hsp70 expression even within the same order (Diptera)

promoters, hsp70, hsp83, luciferase assay, Diptera