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Vol 56(2022) N 1 p. 72-89; DOI 10.1134/S0026893322010022 Full Text

S.O. Galkin1,2, A.N. Anisenko1,2,3, O.A. Shadrina2,3, M.B. Gottikh2,3*

Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family

1Bioengineering and Bioinformatics Department, Moscow State University, Moscow, 119991 Russia
2Chemistry Department, Moscow State University, Moscow, 119991 Russia
3Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119991 Russia

*gottikh@belozersky.msu.ru
Received - 2021-05-12; Revised - 2021-06-09; Accepted - 2021-06-14

The COVID-19 pandemic caused by the previously unknown SARS-CoV-2 Betacoronavirus made it extremely important to develop simple and safe cellular systems which allow manipulation of the viral genome and high-throughput screening of its potential inhibitors. In this review, we made an attempt at summarizing the currently existing data on genetic engineering systems used to study not only SARS-CoV-2, but also other viruses from the Coronaviridae family. In addition, the review covers the basic knowledge about the structure and the life cycle of coronaviruses.

SARS-CoV-2, COVID-19, replicons, pseudoviruses



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