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Vol 55(2021) N 4 p. 487-500; DOI 10.1134/S0026893321030109 Full Text

A.N. Sinyakov1*, V.A. Ryabinin1, E.V. Kostina1

Application of Array-Based Oligonucleotides for Synthesis of Genetic Designs

1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia

*sinyakov@niboch.nsc.ru
Received - 2020-11-19; Revised - 2021-02-08; Accepted - 2021-02-13

The application of array-based oligonucleotides in biological studies is described. These oligonucleotides are mainly used to design large libraries of various nucleotide sequences, which are applied to study protein-nucleic acid interactions, splicing, transcription, translation, and other regulatory processes in mammalian, yeast, and bacterial systems. The application of gene libraries generated by array-based nucleotides along with advanced methods of the combination of DNA duplexes will make it possible to obtain complex genetic designs for synthetic biology.

oligonucleotides, pool, microarrays, gene synthesis, cloning, high-throughput sequencing



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