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Vol 55(2021) N 2 p. 169-183; DOI 10.1134/S0026893321020096  O.A. Kladova1, N.A. Kuznetsov1, O.S. Fedorova1* Initial stages of DNA Base Excision Repair in Nucleosomes 1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia*fedorova@niboch.nsc.ru Received - 2020-07-29; Revised - 2020-08-21; Accepted - 2020-08-26 In mammalian cells, base excision repair (BER) is the main pathway responsible for the correction of a variety of chemically modified DNA bases. DNA packaging in chromatin affects the accessibility of damaged sites to the enzymes involved in repair processes. This review presents data concerning the enzymes involved in BER. Within the nucleosome core particle (NCP), the accessibility of damaged DNA to enzymes is hindered by the presence of a histone octamer. This means that the removal of DNA lesions largely depends on their rotational and translational positioning in the NCP, as well as on the specific features of each enzyme. DNA repair, nucleosome, DNA-glycosylase, AP-endonuclease |
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