Vol 54(2020) N 6 p. 894-903; DOI 10.1134/S002689332006014X
M.M. Yurinskaya1, D.G. Garbuz2, V.N. Afanasiev1, M.B. Evgen'ev1,2*, M.G. Vinokurov1
Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia
2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
Received - 2020-05-06; Revised - 2020-05-20; Accepted - 2020-05-26
The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Ywas studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.
GYY4137, Hsp70, lipopolysaccharide, SH-SY5Y, reactive oxygen species, NO, endocytosis