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Vol 54(2020) N 2 p. 269-273; DOI 10.1134/S0026893320020053  Yu.M. Khodarovich1*, D.D. Rakhmaninova1, A.M. Barishnikova1, S.M. Deyev1 Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia*khodarovich@mail.ru Received - 2019-10-10; Revised - 2019-10-10; Accepted - 2019-11-05 A synthetic genetic chain was created-a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline. synthetic biology, gene chains, integrator, transactivator, gene therapy, oncology |
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