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Vol 53(2019) N 3 p. 452-459; DOI 10.1134/S002689331907001X Full Text

Z. Luo1, W. Fang1, C.L. Wang2,3,4*, W.Q. Ma1**

Double Labeling and Simultaneous Monitoring for Hsp70 and Hsf-1 Kinetics in SCC-25 Cells with a Short-Term Dietary Restriction of Leucine Following Heat Shock

1Stomatological Hospital, Southern Medical University,Guangzhou, 510280 China
2Department of Stomatology, Yancheng Hospital, Medical School of Southeast University, Yancheng, 224001 China
3Department of Stomatology, The Third People's Hospital of Yancheng, Yancheng, 224001 China
4Department of Stomatology, The Sixth Affiliated Hospital of Nantong University, Yancheng, 224001 China

*wangchanglin89@outlook.com
**Maweiqun0504@outlook.com
Received - 2018-11-07; Revised - 2019-01-31; Accepted - 2019-02-01

To develop a quantum-dot-based multiplexed imaging system for the simultaneous monitoring of Hsf- 1/Hsp70 after heat shock, and to evaluate the effects of combined thermotherapy and leucine deprivation therapy on Hsf-1 inactivation. SCC-25 cells were leucine starved for 0, 1, 2, 3 or 4 days following which the cells underwent heat shock at 42°C for 30 min. At 6 h after heat shock, Hsf-1 activation and translocation to the nucleus was observed in cells that were leucine starved for 0, 1 and 2 days, and the synthesis of Hsp70 and Hsf-1 reached their maximum values and had a tendency to gather in the nucleus. However, in cells that were leucine starved for 3 and 4 days, Hsf-1 activity and Hsp70 synthesis level was dramatically decreased. Dietary restriction of leucine for at least three days could result in the inactivation of Hsf-1, leading to a reduction in Hsp70 synthesis. The combination of thermotherapy and short-term leucine deprivation therapy may become effective approach for the treatment of oral tumors.

oral squamous cell carcinoma, Hsp70, Hsf-1, heat shock



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