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Vol 53(2019) N 3 p. 384-392; DOI 10.1134/S002689331903004X Full Text

J.N. Chen1, Y. Chen1, Y.Y. Wei1, M.A. Raza2, Q. Zou1, X.Y. Xi1, L. Zhu3, G.Q. Tang3, Y.Z. Jiang1*, X.W. Li3**

Regulation of m6A RNA Methylation and Its Effect on Myogenic Differentiation in Murine Myoblasts

1Department of Zoology, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014 China
2Department of Crop Cultivation and Farming System, College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, 611130 China
3Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130 China

*jiangyz04@163.com
**xuewei.li@sicau. edu.cn
Received - 2018-08-31; Revised - 2018-11-03; Accepted - 2018-11-10

N6-methyladenosine (m6A) has been identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, and plays important biological roles in the regulation of cellular metabolic processes. However, its role in myogenic differentiation is unclear. Here, we altered the m6A RNA methylation level by overexpression of METTL3, and explored the effect of m6A RNA methylation on myogenic differentiation of murine myoblasts in vitro. The m6A RNA methylation level is regulated by exogenous methylation inhibitor cycloleucine (Cyc) and methyl donor betaine (Bet). Therefore, chemical reagents of Cyc and Bet were used to test the regulatory effect of m6A RNA methylation on myogenic differentiation. Results showed that METTL3 and Bet positively regulated the m6A RNA methylation levels, and Cyc negatively regulated m6A RNA methylation levels. In addition, m6A methylation positively regulated myogenic differentiation in murine myoblasts. These findings provide insight in the mechanisms underlying the effect of m6A RNA methylation on myogenesis.

m6A RNA methylation, METTL3, myogenic differentiation



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