2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 48(2014) N 3 p. 340-346; DOI 10.1134/S0026893314030170 Full Text

A.V. Rozhkova1*, M.V. Zinovyeva2, A.V. Sass2, I.B. Zborovskaya3, S.A. Limborska1, L.V. Dergunova1

Expression of Sphingomyelin Synthase 1 (SGMS1) Gene Varies in Human Lung and Esophagus Cancer

1Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia
2Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia
3Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478, Russia

Received - 2013-07-19; Accepted - 2013-11-01

The investigation of molecular mechanisms contributing to cancer progression is the burning problem of current research. Considerable attention has been focused on the study of gene expression in cancer cells. Sphingomyelin synthase 1 gene (SGMS1) is one of the genes, the expression of which can be altered in cancer. SMS1 enzyme encoded by this gene catalyzes synthesis of sphingomyelin and diacylglycerol from phosphatidylcholine and ceramide. SMS1 may maintain the balance between cell death and survival by regulating the formation of the proaptotic mediator ceramide and anti-apoptotic mediator diacylglycerol. In addition, changes in the sphingomyelin level and sphingomyelin synthase activity have been observed in cancers of many tissues. However, the peculiarities of SGMS1 gene transcription have been insufficiently explored. In this work, the expression of transcripts of SGMS1 has been investigated by the method of real-time PCR in matched pairs of samples of human lung and esophagus cancer and adjacent tissues without pathology. A significant decrease in SMS1 transcript expression has been found in the samples of human lung cancer. At the same time, in the samples of human esophagus cancer and the adjacent tissues, the expression of SMS1 transcripts varies insignifi- cantly, i.e., it is increased in seven and decreased in five of the fifteen samples. The obtained results indicate that SGMS1 gene is expressed differently in cancers of different genesis.

human sphingomyelin synthase1, human lung cancer, human esophagus cancer, gene expression, transcription level, real-time PCR