JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 53(2019) N 1 p. 38-44; DOI 10.1134/S0026893319010187 Full Text

D.L. Yang1, M.L. Gan1, Y. Tan1, G.H. Ge1, Q.Li2, Y.Z. Jiang3, G.Q. Tang1, M.Z. Li1, J.Y. Wang4, X.W. Li1, S.H. Zhang1*, L. Zhu1**

MiR-222-3р Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2

1Farm Animal Genetics Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130 China
2Sichuan Province General Station of Animal Husbandry, Chengdu, 611130 China
3College of Life and Science, Sichuan Agricultural University, Chengdu, 611130 China
4Chongqing Academy of Animal Sciences, Chongqing, 402460 China

*zhangshl919@163.com
**zhuli7508@163.com
Received - 2018-01-21; Revised - 2018-04-30; Accepted - 2018-05-08

MiR-222-3р has been implicated in tumor cell proliferation and has an important role in the differentiation and maturation of myogenic cells. However, its role in skeletal myoblast proliferation is still unclear. In this study, we found that miR-222-3р expression increases initially and then decreases during C2C12 myoblast proliferation. Using synthetic miRNA mimics and inhibitors in gain- or loss-of-function experiments, we snowed that miR-222-3р overexpression in C2C12 cells promotes myoblast proliferation and represses myofiber formation, while miR-222-3р downregulation has the opposite effect. Using a prediction program, BTG2 was identified as a possible target gene of miR-222-3р. During myogenesis, miR-222-3р mimics repress BTG2 expression, while miR-222-3р inhibitors promote BTG2 expression. Using dual-luciferase reporter assay, we further demonstrated that miR-222-3р specifically targets BTG2. Additionally, we show that siRNA-mediated downregulation of BTG2 expression in C2C12 myoblasts promotes the proliferation and suppresses differentiation. In conclusion, we provide a novel insight into the mechanism by which miR-222-3р regulates the proliferation and differentiation of C2C12 myoblasts by targeting BTG2. This information contributes to our understanding of the role of miRNAs in skeletal muscle development.

MiR-222-3р, BTG2, C2C12 myoblasts, proliferation, differentiation



JMB-FOOTER RAS-JOURNALS