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Vol 52(2018) N 5 p. 660-667; DOI 10.1134/S0026893318050059 Full Text

G.-Q. Bai1*, Y. Jia2, W.-M. Li1, H. Chen1, B. Li1, S.-F. Li1**

De novo Assembly and Analysis of Sumac (Toxicodendron vernicifluum (Stokes) F.A. Barkley) Transcriptomes Provides Insights into the Biosynthesis of Urushiol

1Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Institute of Botany of Shaanxi Province, Xi'an, 710061 China
2Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, 710069 China

*bgq@ms.xab.ac.cn
**lisf60@sina.com
Received - 2017-09-01; Accepted - 2017-12-14

Sumac is universally known for its abundance of raw lacquer. Toxicodendron vernicifluum (Stokes) F.A. Barkley is one of the widely distributed native sumac cultivars. To accelerate sumac breeding for more prolific, high-quality, and robust cultivars, it is essential to explore its lacquer metabolism. However, transcriptomic and genomic data available for sumac are still limited. In this study, we generated the transcriptomic profiles of triploid Toxicodendron vernicifluum CV. Dahongpao (Dahongpao) and diploid T. vernicifluum and Toxicodendron vernicifluum CV. Huoyanzi (Huoyanzi), with 87856 unigenes. About 53% of these unigenes were annotated using Nr, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups (COG) and Gene Ontology (GO). We identified nine differentially expressed candidate genes associated with type III polyketide synthase formation, which is the first step in urushiol biosynthesis. Additionally, a number of simple sequence repeats (EST-SSRs) were identified in T. vernicifluum for further molecular marker-assisted breeding. This study is the first report of Toxicodendron species transcriptome.

differential expression, functional annotation, Illumina sequencing, Toxicodendron vernicifluum, transcriptome



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