JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 48(2014) N 6 p. 767-777; DOI 10.1134/S002689331406003X Full Text

A.G. Bogomolov1,2*, T.V. Karamysheva1, N.B. Rubtsov1,2

Fluorescence in situ hybridization with DNA probes derived from individual chromosomes and chromosome regions

1Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia
2Novosibirsk State University, Novosibirsk, 630090 Russia

*mantis-anton@gmail.com
Received - 2014-06-09; Accepted - 2014-07-03

Repetitive DNA accounts for a significant part of the eukaryotic genome, forming large clusters or being relatively uniformly distributed through euchromatic chromosome regions. Repeats make it difficult to analyze and to identify chromosomal material by its DNA composition via fluorescence in situ hybridization (FISH). In most cases, signals from unique sequences are required for identifying the chromosome regions by FISH. The review considers the feasibility, advantages, and disadvantages of conventional methods to suppress repetitive DNA hybridization, methods to construct repeat-free probes, and methods to estimate the signal intensity for chromosome-specific DNA sequences via image processing in the case of multicolor FISH. The efficiency of different techniques used to generate the DNA probes, different FISH protocols, and the processing of microscopic images depends on the genome size and structure in the species under study. Hence, several approaches to the analysis are discussed for species with extremely large genomes, rare species, and species where genomic and Cot-1 DNAs are difficult to obtain in the amounts required for suppression of repetitive DNA hybridization.

repetitive DNA, chromosome-specific DNA sequences, chromosome painting, FISH, CISS hybridization, microscopic image enhancement



JMB-FOOTER RAS-JOURNALS