2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 52(2018) N 3 p. 295-305; DOI 10.1134/S0026893318020097 Full Text

A. Panov*

Perhydroxyl Radical (HO2) as Inducer of the Isoprostane Lipid Peroxidation in Mitochondria

Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630117 Russia

Received - 2017-02-10; Accepted - 2017-04-14

The nonenzymatic isoprostane pathway of lipid peroxidation of polyunsaturated fatty acids results in formation of products, termed isoprostanes, which have very large positional and stereo isomerism, possess various biological activities, produce adducts with proteins, and thus contribute to pathogeneses of the age-dependent diseases. However, it was unclear what mechanism drives this type of lipid autoxidation, and why the products have very large isomerism. We propose a mechanism when perhydroxyl radicals (HO2) react with polyunsaturated fatty acids in the hydrophobic milieu of membranes. In the membrane HO2 initiates a chain of reactions with formation first H2O2, which undergoes homolytic fission producing two OH radicals, thus very rapidly abstracting three H atoms from a polyunsaturated fatty acid. As a result, the HO2 molecule is converted to two molecules of water, and the molecule of a polyunsaturated fatty acid loses two double bonds, becomes highly unstable and undergoes peroxidation and random intramolecular re-arrangements causing a very large isomerism of the final products. The extremely high reactivity of 2 with polyunsaturated fatty acids is the cause of very subtle and slow accumulation of damages in the membrane and membrane associated proteins, even though the concentration of 2 relative to superoxide radical may be very low.

lipid peroxidation, superoxide radical, perhydroxyl radical, mitochondria, isoprostanes, oxidative stress