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Vol 48(2014) N 5 p. 727-733; DOI 10.1134/S0026893314050082 Full Text

M.K. Kukhanova1*, A.N. Korovina1, Yu.A. Sharkin2, A.V. Azhayev2, S.N. Kochetkov1

2'-Fluoronucleotides as substrates of viral replicative polymerases

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
2East Finland University, Kuopio, 70211, Finland

*kukhan86@hotmail.com
Received - 2014-05-14; Accepted - 2014-05-19

Substrate specificities of three viral replicative polymerases of different origins (HIV reverse transcriptase, hepatitis C virus RNA polymerase, and herpes virus DNA polymerase) towards 2'F-NTP were studied. Activated DNA, polyA-oligoU6, and (2'F-A)20-oligoU6 complexes were used as templates. It was shown that all DNA polymerases studied incorporate 2'F-NMP into the 3'-end of primer-template complexes. HIV reverse transcriptase and herpes virus DNA polymerase can elongate synthesis with both dNTP and 2'F-NTP. Homopolymer (2'F-A)20 can serve as a template for the polymerization of UTP as well as 2'F-UTP catalyzed by hepatitis C virus polymerase although with the efficacy about five-to tenfold lower compared to the natural primer-template complex. The pyrophosphorolysis reaction of 2'F-CMP residue on the 3'-end of the primer catalyzed with HIV reverse transcriptase proceeds with an efficiency that is two orders of magnitude less compared to the natural dNMP residues in the same system.

2'-fluoro-nucleotides, viruses, DNA polymerases, RNA polymerases, pyrophosphorolysis



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