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Vol 48(2014) N 5 p. 709-7717; DOI 10.1134/S0026893314050124 Full Text

A.V. Sorokan, B.R. Kuluev, G.F. Burkhanova, I.V. Maksimov*

RNA Silencing of the Anionic Peroxidase Gene Impairs Potato Plant Resistance to Phytophthora infestans (Mont.) de Bary

Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa, 450054 Russia

*maksimov@ufaras.ru
Received - 2014-03-11; Accepted - 2014-04-24

Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment were obtained by agrobacterial transformation. A manifold decrease in activity of anionic isoperoxidase with pI ~ 3.5 in the transformed plants demonstrated that the enzyme is encoded by M21334. The transformed plants showed a decrease in lignin accumulation and a dramatically lower resistance to the late blight agent Phytophthora infestans, implicating the enzyme in the response to P. infestans infection.

Solanum tuberosum, Phytophthora infestans, peroxidase, agrobacterial transformation, lignification



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