2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 45(2011) N 6 p. 941-949;
V.V.Stavchansky1*, T.V.Tvorogova2, A.V.Botsina2, V.I.Skvortsova2, S.A.Limborska1,2, N.F.Myasoedov1, L.V.Dergunova1,2

Effect of Semax and Its C-terminal Peptide PGP on Expression of Neurotrophins and Their Receptors in Rat Brain during Incomplete Global Ischemia

1Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia
2Federal Stroke Institute, Russian State Medical University, Moscow, 117997, Russia

Received - 2010-12-20; Accepted - 2011-03-11

Neurotrophins regulate key functions of nervous tissue cells. Analysis of neurotrophin mRNA expression is an appropriate tool to assess therapeutic efficiency of antistroke drugs. We have analyzed the effect of synthetic peptide semax and its C-terminal Pro-Gly-Pro tripeptide on mRNA expression of neurotrophins Ngf, Bdnf, and Nt-3 and their receptors TrkA, TrkB, TrkC, and p75 in rat frontal cortex, hippocampus, and cerebellum after bilateral common carotid artery occlusion. The animals were decapitated at 30min and 1, 2, 4, 8, 12, and 24 h after the operation. The mRNA expression of neurotrophins and their receptors was assessed by relative quantification using real-time RT-PCR. Our results demonstrated that ischemia caused a significant decrease in gene expression in the hippocampus. Semax and PGP treatment affected the expression of neurotrophins and their receptors predominantly in the frontal cortex and hippocampus of the ischemized animals. In the frontal cortex, Semax treatment resulted in a decrease of mRNA level of neurotrophin receptors, while PGP treatment increased the level of these mRNA. Maximal neuroprotective effect of both peptides was observed in the hippocampus 12 h after occlusion. A decrease of gene expression of neurotrophins and their receptors caused by the occlusion was overcome by Semax and PGP. These results clarify the mechanism of Semax action and reveal certain features of mRNA expression of neurotrophins and their receptors under experimental conditions.

BDNF, NGF, Nt-3, TrkA, TrkB, TrkC, p75, Semax, PGP, real-time qRT-PCR, Wistar rat, incomplete global cerebral ischemia, mRNA expression