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Vol 51(2017) N 4 p. 586-591; DOI 10.1134/S0026893317040082 Full Text

M.D. Chanyshev1,2*, D.S. Ushakov1,2, L.F. Gulyaeva1,2,3

Expression of miR-21 and Its Acat1, Armcx1, and Pten Target Genes in Liver of Female Rats Treated with DDT and Benzo[a]pyrene

1Institute of Molecular Biology and Biophysics, Novosibirsk, 630117 Russia
2Novosibirsk State Pedagogical University, Novosibirsk, 630126 Russia
3Novosibirsk State University, Novosibirsk, 630090 Russia

*chanish@mail.ru
Received - 2016-07-11; Accepted - 2016-10-13

MiR-21 is the most studied cancer-promoting oncomiR, which target numerous tumor suppressor genes associated with proliferation, apoptosis, and invasion. Here we have studied the synthesis of miR-21 and quantified the mRNA and protein levels for miR-21 potential target genes, i.e., Acat1, Armcx1, and Pten, in the livers of female Wistar rats after their treatment with either 1,1-trichloro-2,2-di(4-chlorophenyl)ethane (DDT) or benzo[a]pyrene (BP). The most important finding appears to be the significant decrease in the miR-21 level the day after treatment with DDT with subsequent rebound. These changes are accompanied by an increase and subsequent drop in the levels of mRNAs and proteins of the Acat1, Armcx1, and Pten genes. These observations indicate the involvement of miR-21 in the posttranscriptional regulation of the Acat1, Armcx1, and Pten genes in response to xenobiotics. We hypothesize that the toxic effects of xenobiotics may be indirect and may manifest by inducing epigenetic changes, particularly through the regulation of miRNAs and their target genes.

miR-21, Acat1, Armcx1, Pten, DDT, benzo[a]pyrene, target genes



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