2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 51(2017) N 4 p. 555-561; DOI 10.1134/S0026893317030062 Full Text

Y. Huang1,2, S.-H. Liang1,2, L.-B. Xiang1,2, X.-T. Han1,2, W. Zhang1,2, J. Tang1,2, X.-H. Wu1,2, M.-Q. Zhang1,2*

miR-218 Promoted the Apoptosis of Human Ovarian Carcinoma Cells via Suppression of the WNT/β-Catenin Signaling Pathway

1Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032 China
2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China

Received - 2016-07-05; Accepted - 2016-09-08

MicroRNA-218 (miR-218) is a short, noncoding RNA, with multiple biological functions. In this study, we aimed to investigate the potential effects of miR-218 on the apoptosis of human ovarian carcinoma cells and the underlying mechanisms by which miR-218 exerted its actions. After over-expressing miR-218 in human ovarian carcinoma (OVCAR3) cells, cell viability was determined by MTT method, cell apoptosis was observed by flow cytometry (FCM), mRNA expression of miR-218, Bcl2, Bax was measured by RT-PCR and protein expression levels of Wnt, tankyrase and β-catenin were quantified by Western blots. Over-expression of miR-218 potently suppressed cell viability and promoted the apoptosis of human ovarian carcinoma cells in a time-dependent manner. In addition, the down-regulation of tankyrase expression level was detected in miR-218-over-expressed cells. Following the block of the Wnt/β-catenin signaling pathway using the inhibitor XAV-939, the effects of miR-218 on the proliferation and apoptosis of human ovarian carcinoma cells were significantly suppressed. Augmenting expression of miR-218 and/or miRNA-218 mimicking therapeutics may provide viable avenue for the treatment of ovarian cancer.

miR-218, human ovarian carcinoma cells, proliferation, apoptosis, Wnt/β-catenin