Vol 51(2017) N 4 p. 555-561; DOI 10.1134/S0026893317030062
Y. Huang1,2, S.-H. Liang1,2, L.-B. Xiang1,2, X.-T. Han1,2, W. Zhang1,2, J. Tang1,2, X.-H. Wu1,2, M.-Q. Zhang1,2*
miR-218 Promoted the Apoptosis of Human Ovarian Carcinoma Cells via Suppression of the WNT/β-Catenin Signaling Pathway1Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032 China
2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032 China
Received - 2016-07-05; Accepted - 2016-09-08
MicroRNA-218 (miR-218) is a short, noncoding RNA, with multiple biological functions. In this study, we aimed to investigate the potential effects of miR-218 on the apoptosis of human ovarian carcinoma cells and the underlying mechanisms by which miR-218 exerted its actions. After over-expressing miR-218 in human ovarian carcinoma (OVCAR3) cells, cell viability was determined by MTT method, cell apoptosis was observed by flow cytometry (FCM), mRNA expression of miR-218, Bcl2, Bax was measured by RT-PCR and protein expression levels of Wnt, tankyrase and β-catenin were quantified by Western blots. Over-expression of miR-218 potently suppressed cell viability and promoted the apoptosis of human ovarian carcinoma cells in a time-dependent manner. In addition, the down-regulation of tankyrase expression level was detected in miR-218-over-expressed cells. Following the block of the Wnt/β-catenin signaling pathway using the inhibitor XAV-939, the effects of miR-218 on the proliferation and apoptosis of human ovarian carcinoma cells were significantly suppressed. Augmenting expression of miR-218 and/or miRNA-218 mimicking therapeutics may provide viable avenue for the treatment of ovarian cancer.
miR-218, human ovarian carcinoma cells, proliferation, apoptosis, Wnt/β-catenin