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Vol 44(2010) N 3 p. 479-487;
N.N. Koroleva, P.V. Spirin, A.V. Timokhova, P.M. Rubtsov, S.N. Kochetkov, V.S. Prasolov, S.N. Beljelarskaya*

Baculovirus Vectors for Efficient Gene Delivery and Expression in Mammalian Cells

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia

*belj@eimb.ru
Received - 2009-10-06; Accepted - 2009-11-19

Baculovirus expression vectors are extensively used for the delivery of foreign genes and expression of recombinant proteins in insect and mammalian cells. Modified baculoviruses containing mammalian promoter elements (BacMam viruses) for an efficient transient and stable transduction of diverse mammalian cells ensure a high level of heterologous protein expression both in vitro and in vivo. Recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus promoter, green or red fluorescent protein gene, SV40pA polyadenylation signal, and polylinker MCS were constructed for the delivery of genes encoding hepatitis C virus structural proteins into mammalian cells. In HEK293T and Huh7 cells, formation of glycoprotein complexes and HCV-like particles was observed. A high efficiency of the baculovirus-mediated gene transfer and expression of the virus envelope proteins in mammalian cells was demonstrated using fluorescence, flow cytometry, and immunoblot techniques.

baculovirus AcMNPV, BacMam virus, hepatitis C virus (HCV), HCV structural proteins, HCV-like particles, mammalian cells HEK293T, COS-7 and Huh7, Sf9 insect cells



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