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Vol 45(2011) N 2 p. 283-290;
A.A. Kashevarova*, E.N. Tolmacheva, E.A. Sazhenova , N.N. Sukhanova, I.N. Lebedev

Epigenetic Status of Cell Cycle Regulation Genes in the Placenta of Human Embryos with Chromosomal Mosaicism

Institute of Medical Genetics, Tomsk Research Center, Siberian Division, Russian Academy of Medical Sciences, Tomsk, 634050 Russia

*anna.kashevarova@medgenetics.ru
Received - 2010-06-01; Accepted - 2010-06-05

Epigenetic mechanisms of cell cycle regulation providing for maintenance of genome stability and precise transmission of hereditary information to the daughter cells attracts considerable interest. State-of-the-art molecular technologies allow the genome-wide methylation pattern to be determined in a single experiment. In this work, we pioneered in determining the epigenetic status of the placental tissues of the human embryos with mosaic karyotype using a genome-wide Illumina Infinium HumanMethylation27 BeadChip array (Illumina, United States), comprising 27578 CpG dinucleotides contained in 14475 genes. The groups of genes associated with the cell cycle and its regulation that contain differentially methylated CpG sites in their promoter regions have been determined. It was demonstrated that the methylation level most frequently changed in oncogenes (ARHGEF1 and FGF5), tumor suppressors (APC2, BRACA2, DCC, GRLF1, RB1, TP73, TSPYL2, and VHL), and the genes involved in the regulation of chromosome segregation (CNTROB, GMNN, PROCR, and TACC1).

epigenetic regulation, genome-wide Infinium HumanMethylation27 BeadChip array, oncogenes, tumor suppressors, chromosome segregation, chromosomal mosaicism, human embryos



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