2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 45(2011) N 2 p. 190-196;
Y.T. Huai1, X.Y. Lan1, L. Ma1, J. Wang1, C.Z. Lei1, H. Chen1*, J.Q. Wang2

Novel mutation in TGA stop-codon of bovine SIX6 gene

1College of Animal Science and Technology, Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100, China
2The Research Center of Beef Engineering and Technology in Henan, Zhengzhou, 450003, China

Received - 2010-01-26; Accepted - 2010-03-22

As a transcriptional regulatory gene of the SIX family, SIX6 (also known as OPTX2, SIX9), probably affects pituitary development and secretion of hormones, suggesting that this gene is a potential candidate gene for studying association with growth trait in animals. Therefore, this study is first of all focused on detecting sequence variations in a bovine SIX6 gene and on its effects on growth traits in 1087 cattle from five Chinese cattle breeds using DNA sequencing and HhaI-ACRS-PCR methods. Herein, a novel mutation (NC_007308: g 2015T>C) in the TGA stop-codon of a bovine SIX6 gene was found, which leads to an ORF shift and extension of the encoded protein for four amino acids (Arg223-Gln224-Arg225-Val226). The frequency of allele "C" varied from 0.255 (Chinese Holsteins) to 0.614 (Hasake). They all were in the Hardy-Weinberg equilibrium except Jiaxian Red and Hasake cattle. Using amino acid sequence alignment and online prediction software, a new helix in the C-terminal domain of the mutated bovine SIX6 protein was revealed, which possibly affects pituitary development and hormone secretion. So, relationship analysis between this polymorphism and growth traits in the Nanyang breed was carried out based on a proper linear model. Although no statistically significant associations were observed (P>0.05), the presented work preliminarily demonstarted a novel mutation in the TGA stop-codon which extends the spectrum of genetic variations of the bovine SIX6 gene and might be of interest in terms of its association with other biophysical and biochemical indexes.

SIX6 gene, stop-codon, mutation, ACRS-PCR, growth traits, cattle