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Vol 42(2008) N 1 p. 117-122;
I.Z. Shukshina1, E.E. Minyat2

Dimerization of short RNA fragments of avian retroviruses as revealed using 2-aminopurine fluorescence

1Moscow Institute of Physics and Technology, Dolgoprudnyi, Moscow Region, 141700, Russia
2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
Received - 2007-12-07; Accepted - 2007-04-03

Dimerization of retroviral RNA is known in detail for several groups of viruses, especially the human immunodeficiency virus (HIV). The dimerization region seems to involve short RNA sequences directly responsible for the formation of dimers of two types, kissing loop-loop (KD) and linear (LD). The 5'-terminal sequences, where dimers are mostly formed, substantially differ between avian retroviruses and HIV, while the mechanisms of their RNA dimerization are the same. RNA dimerization was studied using the adenine analog 2-aminopurine (2-AP), which was incorporated into the loop sequence of short fragments of the avian leucosis virus (ALV) RNA. A temperature dependence of 2-AP fluorescence was used to study the KD formation under various conditions. Magnesium ions and the aminoglycoside antibiotic paromomycin were tested for the effect on KD stability. The highest KD stability was observed at >1 mM Mg2+ and >2.5 μM paromomycin.

RNA, retroviruses, dimerization, structure, fluorescence, 2-aminopurin, antibiotics, paromomycin



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