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Vol 42(2008) N 1 p. 105-109;
A.R. Kayumov1, A.R. Sabirova1, N.P. Balaban1, A.M. Mardanova1, O.N. Ilinskaya1, S.V. Kostrov2, M.R. Sharipova1

Start codon in the Bacillus intermedius gene for serine proteinase

1Faculty of Biology and Soil, Kazan State University, Kazan, Tatarstan, 420008, Russia
2Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 119991, Russia
Received - 2007-04-11; Accepted - 2007-05-03

The translation initiation site was determined for Bacillus intermedius aprBi (AN AY754946), coding for extracellular subtilisin-like serine proteinase secreted at the stationary growth phase. Analysis of the aprBi open reading frame revealed three potential translation start sites (TTG, GTG, and ATG). Using the SignalP online freeware program, the probabilities of their functional activity were evaluated. To identify the translation start, modified subtilisin-like protease genes with nucleotide replacements in putative start codons were obtained by oligonucleotide-directed mutagenesis. The expression of these genetic constructs was investigated in protease-deficient strain B. subtilis AJ73. The results indicated that aprBi translation starts from the alternative GTG codon.

subtilisin-like serine protease, start codon, mutant genes Bacillus intermedius



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