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Vol 47(2013) N 2 p. 316-318;
O.V. Volkova1*, A.V. Panov1, I.A. Kosheleva1,2, T.Z. Esikova1, A.M. Boronin1,2

A New Gene of a TrfA Type Replication Initiator Found in a Caprolactam/Salicylate Degradation Plasmid pBS270

1Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290 Russia
2Pushchino State Educational Institute of Natural Sciences, Pushchino, 142290 Russia

*volkova_o_v@inbox.ru
Received - 2012-10-16; Accepted - 2012-10-29

A mini-replicon was obtained for the caprolactam/salicylate degradation plasmid pBS270 (105kb) of incompatibility group P-7 from Pseudomonas bacteria, and its nucleotide sequence was determined. A new gene that encodes a TrfA-like replication initiator was found on this replicon. The level of homology between this replication initiator and known proteins of the TrfA family suggests that the obtained replicon can be classified as IncP-1-like. The pBS270mini was shown to be chimeric.

Pseudomonas, plasmid pBS270, mini-replicon, nucleotide sequence, trfA, IncP groups



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