Vol 47(2013) N 2 p. 267-274;
N.A. Skryabin1*, E.N. Tolmacheva1, I.N. Lebedev1,2, M.V. Zavyalova2,3, E.M.Slonimskaya2,3, N.V.Cherdyntseva2,3
Dynamics of Aberrant Methylation of Functional Groups of Genes in Progression of Breast Cancer1Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634050 Russia
2Siberian State Medical University, Tomsk, 634050 Russia
3Cancer Research Institute, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634009 Russia
Received - 2012-08-07; Accepted - 2012-10-11
For the first time, the epigenetic status of breast benign proliferative processes, malignant breast tumors, and metastases to regional lymph nodes has been studied using the GoldenGate Cancer Panel I DNA methylation microarray (Illumina, United States). The functional groups of differentially methylated genes were identified in each set of samples. The aberrant methylation of genes that regulate cell proliferation and mobility was found in the samples of benign proliferative breast processes. The aberrant methylation of genes responsible for cell differentiation and proliferation, as well as protein phosphorylation and cell mobility, was observed in the samples of malignant breast tumors. The differential methylation of the genes that regulate cell adhesion, the formation of anatomical structures, angiogenesis, immune response, signal transduction, and protein phosphorylation were found in samples with metastases to regional lymph nodes compared to the unaltered breast epithelium. It was found that tissues that range from benign proliferative processes and metastases to regional lymph nodes were generally characterized by a relatively lower level of epigenetic variability com- pared to the tissues of the primary tumor.
DNA methylation, DNA methylation microarray «GoldenGate Cancer Panel I», breast cancer