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Vol 47(2013) N 2 p. 205-214;
O.V. Volkova1*, A.V. Panov1, I.A. Kosheleva1,2, A.M. Boronin1,2

Classification of IncP-7 Plasmids Based on Structural Diversity of Their Basic Replicons

1Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290 Russia
2Pushchino State Educational Institute of Natural Sciences, Pushchino, 142290 Russia

*volkova_o_v@inbox.ru
Received - 2012-06-30; Accepted - 2012-09-27

The structural diversity of basic replicons and repB gene was analyzed for the first time in a large collection of IncP-7 plasmids by PCR, restriction endonuclease analysis, and partial sequencing. It was found that the DNA fragment that contains the gene for UvrD-like helicase RepB is a part of all known P-7 replicons, but often acts as a hot insertion spot for different IS-elements. Based on the detected divergence of the repA-oriV-parWABC nucleotide sequence, the first system of P-7 plasmid classification has been proposed. Most degradation plasmids were classified in the β subgroup; the streptomycin resistance plasmid Rms148 (IncP-7 archetype) was placed into the α subgroup. The γ subgroup included the carbazole degradation plasmid pCAR1 and NAH/SAL-plasmids from the pY line (Yamal oil deposits), and the CAP plasmid pBS270 with a presumably reduced P-7 replicon was classified into a tentative δ subgroup. It was shown that, in most cases, the character of molecular organization of IncP-7 basic replicons did not correlate with particular phenotypic traits; that is, a given P-7 subgroup can include plasmids that encode different phenotypic markers.

Pseudomonas, IncP-7 group plasmids, basic replicon, restriction, nucleotide sequence, repA-oriV-parWABC polymorphism, classification



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