JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 47(2013) N 1 p. 85-96;
T.B. Kastalyeva1*, N.V. Girsova1, K.A. Mozhaeva1, Ing Ming Lee2, R.A. Owens2

Molecular Properties of Potato Spindle Tuber Viroid (PSTVd) Isolates of the Russian Research Institute of Phytopathology

1Russian Research Institute of Phytopathology (VNIIF), Bolshie Vyaziomy, Moscow region, 143050 Russia
2Molecular Plant Pathology Laboratory (USDA/ARS), Beltsville, MD 20705, USA

*kastalyeva@vniif.ru
Received - 2012-03-28; Accepted - 2012-04-25

The complete nucleotide sequences of more than 100 isolates of Potato spindle tuber viroid (PSTVd) collected from locations in the territory of Russia and the former USSR have been determined. These sequences represent 43 individual sequence variants, each containing 1-10 mutations with respect to the "intermediate" or type strain of PSTVd (GenBank Acc. No. V01465). Isolates containing 2-5 mutations were the most common, and 24 sequence variants are described here for the first time. Twenty one isolates contained a mutation found only in Russian and Ukrainian isolates of PSTVd up till now; i.e., replacement of the adenine at position 121 with cytosine (A121C). Many of these isolates contained two mutations-deletion of one of three adenine residues occupying positions 118-120 plus replacement of the adenine at position 121 with either uracil or cytosine (-A120, A121U/C). Both combinations of mutations were phenotypically neutral, i.e. symptom expression in Rutgers tomato was unaffected. Phylogenetic analysis of the sequences of different PSTVd isolates presented in work together with sequences of other naturally-occurring isolates obtained from Internet databases suggesting that known PSTVd isolates may be divided into four groups: (i) a group of isolates from potato and ornamentals where the type strain of PSTVd (PSTVd.018) may be considered to represent the ancestral sequence, (ii) a second group of isolates from potato and ornamentals where PSTVd.123 play the same role as PSTVd.018 for the first group, and iii) a group of potato isolates where PSTVd.125 is a possible ancestral sequence. The fourth and most divergent group of PSTVd isolates differs significantly from these first three groups. The majority of isolates in this group originate from New Zealand and Australia and infect different solanaceous hosts (tomato, pepper, cape gooseberry, potato, and others).

Potato spindle tuber viroid (PSTVd), viroid primary and secondary structure, viroid pathogenicity, mutation frequency



JMB-FOOTER RAS-JOURNALS