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Vol 46(2012) N 5 p. 731-734; D.O. Fesenko1,2*, O.V. Kalennik1, V.E. Barsky1, A.S. Zasedatelev2, T.V. Nasedkina2 Biochip Development for Determining Y-Haplogroups That Occur in Russian Populations 1OOO Biochip-IMB, Moscow, 119991 Russia2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia *biochip@nm.ru Received - 2011-11-02; Accepted - 2012-02-14 A biochip has been developed that enables one to determine Y-chromosome haplogroups C, DE, G, H, I, J, L, N, O, and R in the DNA sample. SNPs M130, M145, P257, M69, U179, M304, M185, M231, M175, P224 were selected as haplogroup markers, correspondingly. The genotyping included two-round PCR with fluorescent labeling of the product followed by hybridization with immobilized probes on the biochip. An analysis of the ratios of fluorescent signals for immobilized wild-type probe-group specific probe pairs for each of the chosen polymorphic markers showed a high accuracy Y-haplogroup genotyping using the biochip. The reliability of genotyping was confirmed by direct sequencing. genotyping, biochip, Y-chromosome haplogroup, SNP, nested PCR |
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