2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 46(2012) N 5 p. 705-709;
A.D. Perenkov1*, D.V. Novikov1, N.A. Sakharnov1, A.V. Alyasova2, O.V. Utkin1, A.U. Baryshnikov3, V.V. Novikov1

Heterogeneous CD38 Expression in Tumor Tissues of Patients with Colorectal Cancer

1Institute of Molecular Biology and Regional Ecology, Lobachevskii State University of Nizhni Novgorod, Nizhni Novgorod, 603950, Russia
2Nizhni Novgorod State Medical Academy, Nizhni Novgorod, 603005, Russia
3Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478, Russia

Received - 2012-02-10; Accepted - 2012-03-14

The CD38 gene encodes a membrane-bound protein that takes part in cell adhesion and catalyzes the formation of cyclic ADP-ribose. The presence of the full-size and alternative forms of CD38 mRNA in tumor tissue specimens from patients with colorectal cancer and tumor cell lines was analyzed by RT-PCR. It was shown that tumor specimens contained cells that constantly express CD38; full-size CD38 mRNA was detected more frequently than its alternative form. CD38 mRNA was found in Colo-205, T-84, HCT15, and HCT116 cells, but not in Caco-2 or SW-620 cells. CD38 expression was observed in all cases of stage-I colorectal cancer; at stages II, III, and IV, it was significantly less frequent. The frequency of CD38 mRNA detection did not depend on the localization or grade of the tumor, or the presence of metastases. An analysis using meth- ylation-sensitive restriction endonucleases showed that CpG dinucleotides were methylated at the binding sites of the Sp1 transcription factor and retinoic acid receptor in all tumor specimens studied, regardless of the presence or absence of CD38 mRNA. The obtained data suggest that CD38 expression in tumor cells of colorectal cancer patients is heterogeneous.

CD38 protein, CD38 gene, alternative forms of CD38 mRNA, methylation, tumor tissue, cell line, colorectal cancer