Vol 46(2012) N 5 p. 670-677;
A.V. Zhigailov1, E.S. Babailova2, N.S. Polimbetova1, D.M. Graifer2, G.G. Karpova2*, B.K. Iskakov1**
Fragment of mRNA Coding Part Complementary to Region 1638-1650 of Wheat 18S RNA Functions as a Translational Enhancer1Aytkhozhin Institute of Molecular Biology and Biochemistry, Ministry of Education and Science of Kazakhstan, Almaty, 480012, Kazakhstan
2Institute of Chemical biology and Fundamental Medicine of Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090 Russia
Received - 2011-09-22; Accepted - 2011-12-26
The possible involvement of 18S rRNA fragment 1638-1650, including basements of the helices h44 and h28, as well as nucleotides of the ribosomal decoding site in the cap-independent mode of the initiation of the translation of plant ribosomes is studied. This rRNA fragment is shown to be accessible for complementary interactions in the 40S ribosomal subunit. It is found that the sequence that is complementary to the 18S rRNA fragment 1638-1650 is able to enhance the efficiency of the reporter mRNA translation when placed just after the initiation codon. The obtained results indicate that, in the course of the cap-independent mode of the initiation of translation, complementary interactions can occur between the mRNA coding sequence and 18S rRNA fragment in the region of the ribosomal decoding site.
plant ribosomes, cap-independent mode of initiation of translation, mRNA, translational enhancers, 8S rRNA, complementary interactions, oligonucleotides and their reactive derivatives, complementary addressed modification