2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 46(2012) N 5 p. 627-638;
S.P. Chumakov1,2*, Y.E. Kravchenko1,2, P.M. Chumakov1,3,4

Protein Complementation as Tool for Studying Protein-Protein Interactions in Living Cells

1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia
3Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, 44195, USA
4Novosibirsk State University, Novosibirsk, 630090

Received - 2012-01-18; Accepted - 2012-02-10

The association and dissociation of protein-protein complexes play an important role in various processes in living cells. The disruption of protein-protein interactions is observed in various pathologies. The study of the nature of these interactions will contribute to a better understanding of the molecular basis of the pathogenesis of the disease and the development of new approaches to therapy. Now there is a set of methods that allow one to reveal and analyze the interaction of proteins in vitro. However, more accurate data can be obtained by studying protein-protein interactions in vivo. One of a few prospective methods is based on the effect of the complementation of fragments of reporter proteins. These reporter systems are based on the change in the fluorescent properties or enzymatic activity of the proteins that can be measured using colorimetric, fluorescent, or other substrates. The principle of the complementation is widely used to analyze protein interactions, to determine of order of interaction of protein partners in different signaling pathways, as well as in high-performance screening studies for detecting and mapping previously unknown protein-protein interactions. The possibilities of existing complementation reporter systems allow one to solve problems that are far beyond the simple registration of the interactions of two or more proteins.

reporter system, protein-protein interactions, protein complementation assay, SLCA, BiFC