|
Vol 43(2009) N 6 p. 982-989; N.V. Soshnikova1, N.I. Vorobyeva1, A.N. Krasnov1,2, S.G. Georgieva1,3, E.N. Nabirochkina1, Yu.V. Shidlovskii1,2 Novel complex formed by the SAYP transcriptional coactivator 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia2Center of Medical Studies, University of Oslo, Moscow, 119334, Russia 3Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia Received - 2009-04-27; Accepted - 2009-05-13 A multisubunit complex that contains the novel evolutionarily conserved transcription factor SAYP was isolated, and its protein composition was determined. SAYP was found to unite two complexes with different functions in transcription activation, namely, the chromatin-remodeling complex PBAP (SWI/SNF) and the general transcription factor TFIID, which is the main component of the RNA polymerase II preinitiation complex. The new supercomplex contained the total set of PBAP and TFIID subunits. All components of the supercomplex (SAYP, TFIID, and PBAP) are essential for its effective interaction with the promoters of SAYP dependent genes. transcription factors, activation of transcription, promoter, chromatin-remodeling complexes, chromatin immunoprecipitation, eukaryotes |
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
