Journal of Molecular Biology

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Vol 58(2024) N 3 p. 547-556; DOI 10.1134/S0026893324700171

A.F. Arutyunyan¹, M.S. Aksenova¹, A.A. Kostyukov², A.A. Stomakhin¹, D.N. Kaluzhny¹, A.L. Zhuze¹*

Synthesis of a Bisbenzoxazole Analogue of Hoechst 33258 as a Potential GC-Selective DNA Ligand
¹Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
²Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia

^*zhuze@eimb.ru
Received - 2023-11-17; Revised - 2023-12-20; Accepted - 2023-12-20

Using a computer modeling approach, we proposed a structure for a potential GC-specific DNA ligand, which could form a complex with DNA in the minor groove similar to that formed by Hoechst 33258 at DNA AT-enriched sites. According to this model, MBoz₂A, a bisbenzoxazole ligand, was synthesized. The results of spectrophotometric methods supported the complex formation of the compound under study with DNA differing in the nucleotide composition.

DNA, minor-groove ligand, sequence-specificity, Hoechst 33258, bisbenzoxazole structure, synthesis, spectrophotometry, circular dichroism