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Vol 58(2024) N 3 p. 429-438; DOI 10.1134/S0026893324700067 Full Text

S.S. Lukina1, A.M. Burdennyy1*, E.A. Filippova1, L.A. Uroshlev2, I.V. Pronina1, N.A. Ivanova1, M.V. Fridman2, K.I. Zhordania3, T.P. Kazubskaya3, N.E. Kushlinskii3, V.I. Loginov1,4, E.A. Braga1,4**

Methylation of Long Noncoding RNA Genes SNHG6, SNHG12, and TINCR in Ovarian Cancer

1Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia
2Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia
3Blokhin National Medical Research Center of Oncology, Moscow, 115478 Russia
4Medical Genetics Research Center, Moscow, 115522 Russia

*burdennyy@gmail.com
**eleonora10_45@mail.ru
Received - 2023-10-24; Revised - 2023-11-20; Accepted - 2023-11-23

Ovarian cancer (OC) develops asymptomatically and escapes diagnosis until advanced stages, the feature contributing to a higher mortality rate. New prospects of OC diagnosis and treatment have been opened in studies of the gene regulation mechanisms that involve long noncoding RNAs (lncRNAs) and identification of the lncRNA genes that are inhibited via methylation of the promoter region. A set of 122 samples of primary OC tumors was examined by methylation specific real-time PCR to assess the methylation level of the lncRNA genes PLUT, SNHG1, SNHG6, SNHG12, and TINCR. A significant increase in their methylation levels was observed in OC (p < 0.001 by the nonparametric Mann-Whitney test). The methylation levels of SNHG6, SNHG12, and TINCR were found to correlate significantly (p < 0.05) with the stage of the tumor process, the histological grade, and metastasis. Downregulation of SNHG6, SNHG12, and TINCR was detected by real-time RT-qPCR, and a significant correlation between methylation and expression was demonstrated for SNHG6 and TINCR (rs < -0.5, p < 0.001). The respective lncRNA genes were assumed to provide potential epigenetic markers of OC.

ovarian cancer, long non-coding RNA, genes, promoter regions, methylation, PLUT, SNHG1, SNHG6, SNHG12, TINCR



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