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Vol 57(2023) N 5 p. 913-920; DOI 10.1134/S0026893323040088  P.A. Ivanov1,2*, A.V. Lyashko1, S.A. Ionov1,3, D.N. Shcherbinin1, I.A. Rudneva1, A.A. Shilov1, N.I. Bunkova1, M.M. Shmarov1,4, T.A. Timofeeva1 Latex Agglutination as an Alternative to the Hemagglutination Reaction of Influenza Viruses 1National Research Center for Epidemiology and Microbiology named after the Academician N.F Gamalei, Moscow, 123098 Russia2Winogradsky Institute of Microbiology RC Biotechnology, Russian Academy of Sciences, Moscow, 117312 Russia 3Mendeleev University of Chemical Technology, Moscow, 117312 Russia 4Sechenov First Moscow State University, Moscow, 101000 Russia *ivanovpa@mail.ru Received - 2022-12-05; Revised - 2023-01-16; Accepted - 2023-02-12 As an alternative to the classical method of erythrocyte hemagglutination, a latex agglutination assay based on the interaction of influenza viruses with the sialoglycoprotein fetuin immobilized on the surface of polystyrene microspheres has been developed. Twelve influenza A virus strains of different subtypes and two influenza B viruses of different lines were tested. Simultaneous titration of viruses using the classical hemagglutination test and the proposed latex agglutination assay showed similar sensitivity and a high degree of correlation (R = 0.94). The obtained microspheres can be used for titration of viruses that recognize and bind sialylated glycans as receptors. In particular, latex aggregation was also induced by the Newcastle disease virus. influenza viruses, microspheres, fetuin, latex agglutination reaction, hemagglutin, receptor binding specificity |
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