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Vol 43(2009) N 4 p. 596-603; I.A. Mufazalov1,2, D.N. Penkov1, B.V. Chernyak3, O.Y. Pletjushkina3, M.Y. Vyssokikh3, R.V. Chertkova4, M.P. Kirpichnikov4, D.A. Dolgikh4, A.A. Kruglov1,2, D.V. Kuprash1, V.P. Skulachev2,3, S.A. Nedospasov1,3 Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia2Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119899, Russia 3Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Academy of Sciences, Moscow, 119899, Russia 4Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia Received - 2009-03-06; Accepted - 2009-03-06 Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that the substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome C gene expression or its localization inside the cell. These cell lines therefore represent an interesting model for the study of apoptotic signaling and physiological functions of cytochrome C. cytochrome C, mouse embryonic fibroblasts, apoptosis |
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