Journal of Molecular Biology

Year	IMPACT-FACTOR
2023	1,500
2022	1,200
2021	1,540
2020	1,374
2019	1,023
2018	0,932
2017	0,977
2016	0,799
2015	0,662
2014	0,740
2013	0,739
2012	0,637
2011	0,658
2010	0,654
2009	0,570
2008	0,849
2007	0,805
2006	0,330
2005	0,435
2004	0,623
2003	0,567
2002	0,641
2001	0,490
2000	0,477
1999	0,762
1998	0,785
1997	0,507
1996	0,518
1995	0,502

Vol 57(2023) N 3 p. 424-439; DOI 10.1134/S002689332303010X

A.N. Sinyakov¹*, E.V. Kostina¹

DNA Fragment Enrichment for High-Throughput Sequencing
¹Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia

^*sinyakov@niboch.nsc.ru
Received - 2022-11-08; Revised - 2022-12-16; Accepted - 2022-12-16

This review describes the application of oligonucleotides, which are mainly obtained using DNA synthesizers of a new generation (microarray DNA synthesizers), for the enrichment of target genomic fragments. The methods of molecular hybridization, polymerase chain reaction, and CRISPR-Cas9 system for this purpose are considered. Examples of the practical use of the developed methods for research and diagnostic purposes are given.

high-throughput sequencing, oligonucleotides, microarray, amplification, diagnostics, exome, CRISPR-Cas