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Vol 43(2009) N 4 p. 591-595; V.M. Chernov, O.A. Chernova, O.V. Gorshkov, A.A. Mouzykantov Mycoplasma adaptation to stress factors: Nucleotide sequences absent in vegetative forms of Mycoplasma gallisepticum S6 cells are found in nonculturable forms Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, Kazan, 420111, RussiaReceived - 2008-12-17; Accepted - 2009-02-11 The adaptation of Mycoplasma gallisepticum S6 to adverse environmental conditions is associated with the transformation of vegetative cell forms to viable but nonculturable (VBNC) forms. The vegetative and VBNC forms proved to differ in the spectrum of PCR products amplified from pvpA-gene, which codes for the phase-variable cytoadhesion protein. The vegetative forms displayed only one amplicon, which contained one open reading frame (1086 bp) with a high homology (97%) to pvpA-gene of M. gallisepticum R and Pendik. The VBNC forms of M. gallisepticum S6 had additional amplicons, whose open reading frames were absent from the complete database sequence of the mycoplasma genome. A high nucleotide sequence homology (54-55%) between pvpA-gene and the additional pvpA-gene amplicons made it possible to suggest that pvpA-gene provided a source for the formation of new regions within the mycoplasma genome during adaptation to adverse environmental conditions. Mycoplasma gallisepticum S6, adaptation, vegetative and nonculturable forms, pvpA-gene, PCR products, nucleotide sequences |
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