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Vol 43(2009) N 4 p. 586-590; V.A. Furalyov, I.V. Kravchenko, V.O. Popov Small interfering RNA targeting the human myostatin gene Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, RussiaReceived - 2009-01-12; Accepted - 2009-01-30 Ten small interfering RNA to human myostatin gene, selected with two different software programs, were synthesized and tested. Three of them exhibited pronounced biological activity and reduced the amount of myostatin mRNA to 22-27%, compared to control level. These small interfering RNAs stimulated the proliferation of human myoblasts and hindered their differentiation. The obtained small interfering RNAs can be used for the development of new approaches in the therapy of sarcopenia and various myodystrophies. Myostatin, siRNA, RNA-interference, myoblasts |
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